New Environmental and Molecular Mutagenesis Study Findings Have Been Reported by Researchers at Bayer (Classification of in vitro genotoxicants using a novel multiplexed biomarker assay compared to the flow cytometric micronucleus test)
By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Investigators publish new report on Environmental and Molecular Mutagenesis. According to news originating from Berlin, Germany, by NewsRx correspondents, research stated, "Regulatory in vitro genotoxicity testing exhibits shortcomings in specificity and mode of action (MoA) information. Thus, the aim of this work was to evaluate the performance of the novel MultiFlow assay composed of mechanistic biomarkers quantified in TK6 cells after treatment (4 and 24 hr): gH2AX (DNA double strand breaks), phosphorylated H3 (mitotic cells), translocated p53 (genotoxicity), and cleaved PARP1 (apoptosis)."
Our news journalists obtained a quote from the research from Bayer, "A reference dataset of 31 compounds with well-established MoA was studied using the MicroFlow micronucleus assay. A positive call was raised following the earlier published criteria from Litron Laboratories. In the light of our data, these evaluation criteria should probably be adjusted since only 8/11 (73%) nongenotoxicants and 18/20 (90%) genotoxicants were correctly identified. Moreover, there is a need for new in vitro tools to delineate the predominant MoA as in the MicroFlow assay only 5/9 (56%) aneugens and 4/11 (36%) clastogens were correctly classified. In contrast, the MultiFlow assay provides more in-depth information about the MoA and therefore reliably discriminates clastogens, aneugens, and nongenotoxicants. By using a lab-specific, practical threshold for the aforementioned biomarkers, 10/11 (91%) nongenotoxicants and 19/20 genotoxicants (95%), 9/11 (82%) clastogens, and 8/9 (89%) aneugens were correctly categorized, suggesting a clear improvement over the MicroFlow. Furthermore, the MultiFlow markers were benchmarked against established methods to assess the validity of the data. Altogether, these findings demonstrated good agreement between the MultiFlow assay and the benchmarking methods."
According to the news editors, the research concluded: "Finally, p21 may improve class discrimination given the correct identification of 4/4 (100%) aneugens and 2/5 (40%) clastogens."
For more information on this research see: Classification of in vitro genotoxicants using a novel multiplexed biomarker assay compared to the flow cytometric micronucleus test. Environmental and Molecular Mutagenesis, 2017;():. (Wiley-Blackwell - www.wiley.com/; Environmental and Molecular Mutagenesis - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1098-2280)
The news correspondents report that additional information may be obtained from S. Wilde, Investigational Toxicology, Bayer AG, Berlin, Germany. Additional authors for this research include M. Dambowsky, C. Hempt, A. Sutter and N. Queisser (see also Environmental and Molecular Mutagenesis).
The direct object identifier (DOI) for that additional information is: https://doi.org/10.1002/em.22130. This DOI is a link to an online electronic document that is either free or for purchase, and can be your direct source for a journal article and its citation.
Keywords for this news article include: Berlin, Europe, Germany, Genetics, Environmental and Molecular Mutagenesis.
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