Studies from Bayer Update Current Data on Nerve Tissue Proteins (A tripartite mode of action approach for investigating the impact of aneugens on tubulin polymerization)
By a News Reporter-Staff News Editor at Pharma Business Week -- A new study on Proteins - Nerve Tissue Proteins is now available. According to news reporting out of Berlin, Germany, by NewsRx editors, research stated, "Chemical-induced disruption of the cellular microtubule network is one key mechanism of aneugenicity. Since recent data indicate that genotoxic effects of aneugens show nonlinear dose-response relationships, margins of safety can be derived with the ultimate goal to perform a risk assessment for the support of drug development."
Our news journalists obtained a quote from the research from Bayer, "Furthermore, microtubule-interacting compounds are widely used for cancer treatment. While there is a need to support the risk assessment of tubulin-interacting chemicals using reliable mechanistic assays, no standard assays exist to date in regulatory genotoxicity testing for the distinction of aneugenic mechanisms. Recently reported methods exclusively rely on either biochemical, morphological, or cytometric endpoints. Since data requirements for the diverse fields of application of those assays differ strongly, the use of multiple assays for a correct classification of aneugens is ideal. We here report a tripartite mode of action approach comprising a cell-free biochemical polymerization assay and the cell-based methods cellular imaging and flow cytometry. The biochemical assay measures tubulin polymerization over time whereas the two cell-based assays quantify tubulin polymer mass. We herein show that the flow cytometric method yielded IC values for tubulin destabilizers and EC values for tubulin stabilizers as well as cell cycle information. In contrast, cellular imaging complemented these findings with characteristic morphological patterns. Biochemical analysis yielded kinetic information on tubulin polymerization."
According to the news editors, the research concluded: "This multiplex approach is able to create holistic effect profiles which can be individually customized to the research question with regard to quality, quantity, usability, and economy."
For more information on this research see: A tripartite mode of action approach for investigating the impact of aneugens on tubulin polymerization. Environmental and Molecular Mutagenesis, 2017;():. (Wiley-Blackwell - www.wiley.com/; Environmental and Molecular Mutagenesis - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1098-2280)
Our news journalists report that additional information may be obtained by contacting V. Stock, Bayer AG, Investigational Toxicology, Muellerstraße 178, Berlin, 13353, Germany. Additional authors for this research include A. Sutter, M. Raschke and N. Queisser (see also Proteins - Nerve Tissue Proteins).
The direct object identifier (DOI) for that additional information is: https://doi.org/10.1002/em.22158. This DOI is a link to an online electronic document that is either free or for purchase, and can be your direct source for a journal article and its citation.
Keywords for this news article include: Berlin, Europe, Germany, Tubulin, Biochemicals, Biochemistry, Risk and Prevention, Microtubule Proteins, Nerve Tissue Proteins.
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