Findings from Bristol-Myers Squibb Reveals New Findings on Immunoglobulins (Drug-to-antibody determination for an antibody-drug-conjugate utilizing cathepsin B digestion coupled with reversed-phase high-pressure liquid chromatography analysis)
By a News Reporter-Staff News Editor at Drug Week -- Data detailed on Immunology - Immunoglobulins have been presented. According to news originating from Bloomsbury, New Jersey, by NewsRx correspondents, research stated, "Antibody drug conjugates or ADCs are currently being evaluated for their effectiveness as targeted chemotherapeutic agents across the pharmaceutical industry. Due to the complexity arising from the choice of antibody, drug and linker; analytical methods for release and stability testing are required to provide a detailed understanding of both the antibody and the drug during manufacturing and storage."
Our news journalists obtained a quote from the research from Bristol-Myers Squibb, "The ADC analyzed in this work consists of a tubulysin drug analogue that is randomly conjugated to lysine residues in a human IgG1 antibody. The drug is attached to the lysine residue through a peptidic, hydrolytically stable, cathepsin B cleavable linker. The random lysine conjugation produces a heterogeneous mixture of conjugated species with a variable drug-to-antibody ratio (DAR), therefore, the average amount of drug attached to the antibody is a critical parameter that needs to be monitored. In this work we have developed a universal method for determining DAR in ADCs that employ a cathepsin B cleavable linker. The ADC is first cleaved at the hinge region and then mildly reduced prior to treatment with the cathepsin B enzyme to release the drug from the antibody fragments, This pre-treatment allows the cathepsin B enzyme unrestricted access to the cleavage sites and ensures optimal conditions for the cathepsin B to cleave all the drug from the ADC molecule. The cleaved drug is then separated from the protein components by reversed phase high performance liquid chromatography (RP-HPLC) and quantitated using UV absorbance. This method affords superior cleavage efficiency to other methods that only employ a cathepsin digestion step as confirmed by mass spectrometry analysis."
According to the news editors, the research concluded: "This method was shown to be accurate and precise for the quantitation of the DAR for two different random lysine conjugated ADC molecules."
For more information on this research see: Drug-to-antibody determination for an antibody-drug-conjugate utilizing cathepsin B digestion coupled with reversed-phase high-pressure liquid chromatography analysis. Journal of Chromatography A, 2017;1481():44-52. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.journals.elsevier.com/journal-of-chromatography-a/)
The news correspondents report that additional information may be obtained from M. Adamo, Bristol Myers Squibb, Bloomsbury, NJ 08551, United States. Additional authors for this research include G.Y. Sun, D.F. Qiu, J. Valente, W.K. Lan, H.T. Song, M. Bolgar, A. Katiyar and G. Krishnamurthy (see also Immunology - Immunoglobulins).
Keywords for this news article include: Bloomsbury, New Jersey, United States, North and Central America, Cysteine Endopeptidases, Essential Amino Acids, Enzymes and Coenzymes, Drugs and Therapies, Diamino Amino Acids, Peptide Hydrolases, Basic Amino Acids, Drug Development, Immunoglobulins, Blood Proteins, Cathepsin B, Cathepsins, Immunology, Antibodies, Lysine, Bristol-Myers Squibb.
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