Reports from B.Q. Luan et al Highlight Recent Findings in Nanotechnology (Single Locked Nucleic Acid-Enhanced Nanopore Genetic Discrimination of Pathogenic Serotypes and Cancer Driver Mutations)
By a News Reporter-Staff News Editor at Cancer Weekly -- Investigators discuss new findings in Nanotechnology. According to news reporting originating from Yorktown Heights, New York, by NewsRx correspondents, research stated, "Accurate and rapid detection of single-nucleotide polymorphism (SNP) in pathogenic mutants is crucial for many fields such as food safety regulation and disease diagnostics. Current detection methods involve laborious sample preparations and expensive characterizations."
Financial supporters for this research include International Business Machines Corporation, National Institute of Food and Agriculture, National Human Genome Research Institute, National Institute of General Medical Sciences (see also Nanotechnology).
Our news editors obtained a quote from the research, "Here, we investigated a single locked nucleic acid (LNA) approach, facilitated by a nanopore single-molecule sensor, to accurately determine SNPs for detection of Shiga toxin producing Escherichia coli (STEC) serotype O157:H7, and cancer-derived EGFR L858R and KRAS G12D driver mutations. Current LNA applications that require incorporation and optimization of multiple LNA nucleotides. But we found that in the nanopore system, a single LNA introduced in the probe is sufficient to enhance the SNP discrimination capability by over 10-fold, allowing accurate detection of the pathogenic mutant DNA mixed in a large amount of the wild type DNA. Importantly, the molecular mechanistic study suggests that such a significant improvement is due to the effect of the single-LNA that both stabilizes the fully matched base-pair and destabilizes the mismatched base-pair."
According to the news editors, the research concluded: "This sensitive method, with a simplified, low cost, easy-to-operate LNA design, could be generalized for various applications that need rapid and accurate identification of single-nucleotide variations."
For more information on this research see: Single Locked Nucleic Acid-Enhanced Nanopore Genetic Discrimination of Pathogenic Serotypes and Cancer Driver Mutations. ACS Nano, 2018;12(5):4194-4205. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)
The news editors report that additional information may be obtained by contacting B.Q. Luan, IBM Thomas J Watson Res, Computat Biol Center, Yorktown Hts, NY 10598, United States. Additional authors for this research include X.W. Chen, K. Tian, P. Singh, Z.Y. Yang, K.S. Gates, M.S. Lin, A. Mustapha and L.Q. Gu.
The direct object identifier (DOI) for that additional information is: https://doi.org/10.1021/acsnano.8b01198. This DOI is a link to an online electronic document that is either free or for purchase, and can be your direct source for a journal article and its citation.
Keywords for this news article include: Yorktown Heights, New York, United States, North and Central America, Nanotechnology, International Business Machines Corporation, Technology Companies, Health and Medicine, Oncology, Cancer.
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