Pulse sequence for parallel 2D(H)CACO/3D(H)CA(CO)NH experiment

JNM-ECZR is equipped with one receiver per 2 RF transmitter module. Multiple receiver functionality is included with the addition of optional RF transmitter receiver modules.
JNM-ECZR allows users to easily collect data with multiple receivers. Pulse sequences can easily be adapted with the addition of a few lines of code to collect multiple receiver data without the need to move any cables or modules.
The diagram (left) shows an example of multiple receivers for the parallel collection of 3D (H)CA(CO)NH H detected and 2D (H)CACO C detected multi-dimensional data sets in a single experiment.

H detected 3D (H)CA(CO)NH spectra of C / N labelled ubiquitin
C detected 2D (H)CACO spectra of C / N labelled ubiquitin acquired in parallel with 3D (H)CA(CO)NH spectra

Spectrometer: JNM-ECZ900R
Sample :1mM C / N labelled ubiquitin in 90%H O / 10%D O H detected 3D (H)CA(CO)NH spectra of C/ N labelled ubiquitin

Kupce E, Kay L E, Freeman R (2010) J. Am. Chem. Soc 132: 18008-18011



If you want to see a printed version,
please click this PDF file.PDF 447KB

Jeol Ltd. published this content on 27 October 2017 and is solely responsible for the information contained herein.
Distributed by Public, unedited and unaltered, on 27 October 2017 08:22:07 UTC.

Original documenthttps://www.jeol.co.jp/en/applications/detail/1454.html

Public permalinkhttp://www.publicnow.com/view/A407FF5551C696F9154E68E36F9FE2B5FE0081BA