Shimadzu : New Nexera Mikros, a Micro Flowrate Compatible Liquid Chromatograph Mass Spectrometer, Provides the Pharmaceutical Industry with High Sensitivity, Durability, and Ease of Use

The following is an example of antibody drug analysis in plasma. The plasma spiked with trastuzumab was pretreated using nSMOL Antibody BA Kit.
In the analysis of signature peptides deriving from trastuzumab, Nexera Mikros provides a dynamic range of 0.00763 - 62.5 μg/mL, good linearity with R2 > 0.99, and excellent accuracy of 101.0% (average).

Lipid mediators are bioactive lipids produced in trace quantities in biological body. They are deeply involved in allergic reactions and inflammatory responses. In inflammatory processes, multiple lipid mediators are mutually correlated, and the inflammatory induction or regression reaction is facilitated by the induction or termination of their production. In these processes, a high sensitivity analysis is required in order to correctly evaluate lipid mediators, as their concentrations in biological body fluctuate significantly.
The analysis data shown below is representative for five standard lipid mediators, including leukotriene and HETE. Micro-flow conditions produce a 10-fold increase in sensitivity compared to semi-micro flow rates in both positive and negative mode. Also presented are results for a) 20-HETE, b) 5,6-DiHETE, and c) LTD4, which were spiked in plasma from a trace quantity (50 fg) standard product, as well as d) intrinsic LTD4 in plasma. The lipid mediators a), b), and c) spiked in plasma were confirmed at micro flow rates.