Study Findings on Pro-Opiomelanocortin Are Outlined in Reports from Mitsubishi Tanabe Pharma (RNAi-mediated knockdown of mouse melanocortin-4 receptor in vitro and in vivo, using an siRNA expression construct based on the mir-187 precursor)
By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Peptide Proteins - Pro-Opiomelanocortin are discussed in a new report. According to news reporting originating in Kanagawa, Japan, by NewsRx journalists, research stated, "RNA interference (RNAi) is a powerful tool for the study of gene function in mammalian systems, including transgenic mice. Here, we report a gene knockdown system based on the human mir-187 precursor."
The news reporters obtained a quote from the research from Mitsubishi Tanabe Pharma, "We introduced small interfering RNA (siRNA) sequences against the mouse melanocortin-4 receptor (mMc4r) to alter the targeting of miR-187. The siRNA-expressing cassette was placed under the control of the cytomegalovirus (CMV) early enhancer/chicken b-actin promoter. In vitro, the construct efficiently knocked down the gene expression of a co-transfected mMc4r-expression vector in cultured mammalian cells. Using this construct, we generated a transgenic mouse line which exhibited partial but significant knockdown of mMc4r mRNA in various brain regions. Northern blot analysis detected transgenic expression of mMc4r siRNA in these regions. Furthermore, the transgenic mice fed a normal diet ate 9% more and were 30% heavier than wild-type sibs. They also developed hyperinsulinemia and fatty liver as do mMc4r knockout mice."
According to the news reporters, the research concluded: "We determined that this siRNA expression construct based on mir-187 is a practical and useful tool for gene functional studies in vitro as well as in vivo."
For more information on this research see: RNAi-mediated knockdown of mouse melanocortin-4 receptor in vitro and in vivo, using an siRNA expression construct based on the mir-187 precursor. Experimental Animals, 2016;66(1):41-50 (see also Peptide Proteins - Pro-Opiomelanocortin).
Our news correspondents report that additional information may be obtained by contacting M. Kato, Research Unit, Neuroscience, Mitsubishi Tanabe Pharma Corporation, 1000 Kamoshida, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan. Additional authors for this research include Y.Y. Huang, M. Matsuo, Y. Takashina, K. Sasaki, Y. Horai, A. Juni, S.I. Kamijo, K. Saigo, K. Ui-Tei and H. Tei.
Keywords for this news article include: Asia, Japan, Kanagawa, Genetics, Neuropeptides, Peptide Hormones, Peptide Proteins, Pituitary Hormones, Pro Opiomelanocortin, Pro-Opiomelanocortin, Nerve Tissue Proteins.
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