Shares in the company rose over 4% after the German travel group's primary listing.
TUI's shareholders voted in February to remove Europe's largest travel operator from the London Stock Exchange.
Company CEO Sebastian Ebel said the return to the Prime Standard on Germany's largest stock exchange was an important day for the firm.
He said TUI was now placing a clear focus on one stock exchange listing - instead of two previously.
He added this should raise the visibility and attractiveness of its shares for global investors.
The company has been listed on the London Stock Exchange, the Hanover Stock Exchange and the OTC market of the Frankfurt Stock Exchange since 2014.
That was due to the merger with Britain's TUI Travel.
But the majority of share trading has shifted to Germany.
Which means the company no longer considers a dual listing sensible.
