BRUSSELS (Reuters) - The European Commission on Thursday called on Italy to provide proof against Germany's allegation that Fiat Chrysler (>> Fiat Chrysler Automobiles NV) used illegal exhaust emissions defeat devices, stepping up pressure on the group amid accusations against it in the U.S.

Germany has asked the EU executive to mediate its dispute with Italy, which rejected its allegations of hidden software on the Fiat 500X, Fiat Doblo and Jeep Renegade models that allowed excess diesel emissions.

Germany's motor vehicle authority KBA began testing the vehicles of several manufacturers, including Fiat Chrysler, after Volkswagen's (>> Volkswagen AG) admission of cheating on emissions tests.

An EU source said that testing carried out on one Fiat 500X vehicle at the EU's own vehicle testing laboratory north of Milan showed suspicious emissions behaviour.

Fiat Chrysler declined to comment.

The German KBA motor vehicle authority had previously said it found a device that throttles back the models' exhaust purification system after 22 minutes - just longer than official regulatory tests.

Fiat Chrysler said at the time that its cars conform to current emissions rules and do not contain defeat devices.

In a sign of mounting frustration in Brussels over what EU officials see as governments colluding with the powerful car industry, the Commission said it was now up to Italian authorities to prove no wrongdoing.

"The German authorities have expressed serious concerns," Commission spokeswoman Lucia Caudet told Reuters. "We have repeatedly asked Italian authorities to come forward with convincing answers as soon as possible."

An EU official said Brussels shares Germany's concerns.

The Commission expects to conclude the Italian, German talks on Fiat Chrysler's compliance in coming weeks but it has little power to enforce a settlement.

In a system the Commission is now seeking to overhaul, national watchdogs approve new cars and alone have the power to police manufacturers - though once approved in one country, vehicles can be sold across the bloc.

As part of a widening clampdown on health-threatening nitrogen oxide (NOx) pollution levels in the wake of the VW scandal, the Commission in December began legal action against Germany, Britain and five other EU member states for failing to police emissions test cheating.

The U.S. Environmental Protection Agency on Thursday accused Fiat Chrysler of illegally using hidden software in several vehicles to allow excess diesel emissions to go undetected.

(Additional reporting by Agnieszka Flak in Milan; Editing by Alexandra Hudson)

By Alissa de Carbonnel

Stocks treated in this article : Volkswagen AG, Fiat Chrysler Automobiles NV