EV Biologics Corp. announced that it has successfully generated several mesenchymal stem/stromal cell (MSC) lines for secretome production and further cell line development. The cell banks were isolated from rigorously screened human donor tissue using xeno-free, serum-free culture media for isolation and expansion, after obtaining informed consent (from donors) for research and development.

Analysis of the cell lines confirmed their identity as MSCs according to established criteria. The Company will use these cell banks for production of their secretome, containing extracellular vesicles, including exosomes, as well as other bioactive proteins and RNA. MSC secretome products have demonstrated great potential for a broad range of therapeutic applications, with the greater benefits having been demonstrated using young stem cells such as these.

The gene expression of these cell banks will be further analyzed to identify optimal cell subpopulations for further cell line development. Their secretome products will be analyzed using multi-omics and cell-based assays to identify the bioactive constituents of their composition and to correlate their bioactivity with specific subpopulations.