Pacific Biosciences of California, Inc. announced two new high throughput library preparation kits and workflows optimized for its Revio sequencing system. With these kits, PacBio takes another leap forward in the long-read market by offering customers automated, scalable, and high-performance library preparation solutions with a 40% reduction in costs and a 60 percent decrease in workflow time. In addition, with the HiFi Plex Prep Kit 96 now including 384 validated barcode adapters, customers will have the ability to run 1,536 samples in a single Revio run.

Engineered for efficiency, the new HiFi Prep Kit 96 and HiFi Plex Prep Kit 96 are designed to allow users the ability to automate long-read sequencing workflow steps and streamline the process of preparing, pooling, and loading samples. These capabilities help eliminate bottlenecks related to cost, time, and labor, making them one of the most cost-effective high-throughput long-read library preparation solutions on the market. For microbial, metagenome, and low-pass whole genome sequencing customers, these new offerings will allow them to unlock large projects with organizations of varying budget requirements.

The new kit configurations will also provide customers with a 4-fold reduction in waste and packaging as compared to the previous generation of PacBio library preparation kits. The new HiFi library prep kits are expected to be available for purchase in the first quarter of 2024, with delivery planned early in the second quarter of 2024. Automated protocols will first be available on the Hamilton NGS STAR system, with qualified protocols on additional PacBio Compatible automation platform partners being added in the future.

Kits contain enough volume for customers to develop their own automated methods.